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Analysis of the binding of TFIIIA to nucleosomes reconstituted onto the X. borealis 5S rRNA gene fragment by agarose gel electrophoresis, showing nucleosomes (lanes 1 to 3) or uncomplexed DNA (lanes 4 to 6) in the absence (lanes 1 and 4) or presence (lanes 2, 3, 5, and 6) of increasing amounts of TFIIIA. The nucleosomes used for the reconstitution were isolated from chicken erythrocytes, and the relative molar ratios of TFIIIA are indicated below the gel.
