| Cycle number | Denature | Anneal | Extend |
|---|---|---|---|
| Preincubation | 94 °C 30 s | NA | NA |
| 15 cycles | 94 °C 15 s | 62 °C 30 s | 70 °C 30 s |
| Final extension | NA | NA | 70 °C 5 min |
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