Survival curves for double mutant daf-2(m41); fer-15(b26ts) (DR1993) and infertile control fer-15(b26ts) (DR1830) adults at 25.5°C. The double mutant lived significantly longer than control strains (P < 0.0001, log rank test). The two arrowheads at the top of the graph correspond to days 6 and 10 of the study, the times at which 100% and 95 ± 3% of the control and daf-2 populations were alive, respectively. Wild type (N2), circles; fer-15, inverted triangles; fer-15; daf-2, diamonds. Filled and open symbols represent data from two independent trials. The mean lifespan (±SE) of wild type was 14.3 ± 0.5 d (n = 222); fer-15, 14.9 ± 0.1d(n = 232); and fer-15; daf-2, 27.3 ± 1.7d(n = 235). Maximum lifespans for the strains were 20 d, 22 d, and 41 ± 1 d, respectively.