. 1998 Mar;18(3):1163–1171. doi: 10.1128/mcb.18.3.1163

TABLE 3.

Effect of srb10Δ on repression of UAS_LEU2-HIS3-lacZ by LexA₈₇-Ssn6^a

Protein	Genotype	β-Gal activity^b		Fold repression
Protein	Genotype	−LexAop	+LexAop	Fold repression
LexA	WT	292	232	1.3
	srb10Δ	330	232	1.4
LexA₈₇-Ssn6	WT	370	55	6.7
	srb10Δ	436	253	1.7

Isogenic strains MCY3912 (wild type [WT]) and MCY3668 (srb10Δ) were cotransformed with plasmid pBTM116 or pCK23 (21) expressing LexA and LexA₈₇-Ssn6, respectively, and UAS_LEU2-HIS3-lacZ reporter plasmids with no or one LexA operator 5′ to UAS_LEU2 (Fig. 1).

β-Galactosidase (β-Gal) activity was assayed in permeabilized cells. Values are averages for three to five independent transformants. Standard errors were <17%.