TABLE 5.
Effects of ctk1Δ and srb10Δ on GAL1-lacZ expressiona
| Relevant genotype | β-Gal activityb | Fold induction defect |
|---|---|---|
| Wild type | 2,210 | 1.0 |
| srb10Δ | 871 | 2.5 |
| ctk1Δ | 275 | 8.0 |
| srb10Δ ctk1Δ | 122 | 18 |
a
Strains carrying an integrated GAL1-lacZ fusion gene were derived from the cross of MCY3667 (srb10Δ ctk1Δ) with MCY3659 (wild type), which had been transformed with plasmid pRY171 (64). Cells were grown in rich medium with 2% galactose (inducing conditions).
b
β-Galactosidase (β-Gal) activity was assayed in permeabilized cells. Values are averages for at least three segregants of each genotype. Standard errors were <15%.