Table IV.
Maximum catalytic activity of enzymes measured in growing embryos of VfAAP1-expressing seeds and the wild type
28 DAP; means ± sd; bold values indicate significant differences according to Student's t test of normalized (log-transformed) data; P < 0.05; n = 5 to 7.
| Enzymes | AAP-10 | APP-12 | APP-21 | Wild Type |
|---|---|---|---|---|
| ADP Glc pyrophosphorylase | 0.32 ± 0.06 | 0.44 ± 0.04 | 0.33 ± 0.03 | 0.49 ± 0.01 |
| Suc synthasea | 2.77 ± 0.03 | 2.6 ± 0.19 | 2.38 ± 0.24 | 3.28 ± 0.75 |
| PEP carboxylase | 0.65 ± 0.02 | 0.67 ± 0.07 | 0.74 ± 0.04 | 0.64 ± 0.15 |
| PEP phosphatase | 0.4 ± 0.01 | 0.4 ± 0.1 | 0.4 ± 0.1 | 0.4 ± 0.01 |
| Asp-Aminotransferase | 16.2 ± 0.4 | 17.3 ± 0.8 | 17.4 ± 0.5 | 17.1 ± 0.9 |
| Glc-6-P dehydrogenase | 0.29 ± 0.043 | 0.3 ± 0.057 | 0.25 ± 0.024 | 0.31 ± 0.018 |
| Pyruvate kinase | 1.25 ± 0.14 | 1.43 ± 0.24 | 1.78 ± 0.25 | 1.69 ± 0.15 |
| Citrate synthase | 0.26 ± 0.024 | 0.29 ± 0.015 | 0.29 ± 0.04 | 0.32 ± 0.023 |
a
Micromoles per gram fresh weight min−1.