Figure 2.

Nodulation, mycorrhizal colonization, and growth kinetics of lot1 and the wild type. A, Numbers of nodule primordia and mature nodules as a function of time. One week after transplantation, inoculation of M. loti Tono was carried out as described in “Materials and Methods.” The means and sds are presented (n = 10). B, Comparison of mycorrhizal colonization between lot1 and the wild type. The means and sds (n = 3) are not significantly different at P < 0.01 according to the t test. C to F, Growth kinetics of shoots and roots. The means and sds are presented (n = 10). C and D, Shoot weight and root weight, respectively. Seedlings at 7 d after germination were transplanted onto nitrogen-free B & D medium on vermiculite, and, 1 week later, they were inoculated with M. loti Tono. E and F, The seedlings were transplanted onto nitrogen-rich 0.5× Gamborg B5 medium on vermiculite and then grown without bacteria. White circles and black squares in all sections except B indicate the wild type and lot1 mutant, respectively.