Grafting experiments with lot1 and the wild type (WT). A, Plants grown for 6 weeks after grafting and 5 wpi of M. loti Tono. Bar, 2 cm. B, Nodulation of lot1 roots to which wild-type shoots had been grafted. Bar, 1 cm. C, Nodulation of wild-type roots to which lot1 shoots had been grafted. Bar, 1 cm. D, Number of mature nodules. The means and sds are presented (n = 10, 14, 5, and 17 for lot1/lot1, WT/ lot1, lot1/WT, and WT/WT, respectively). Different letters above the bars indicate significant differences at P < 0.05 according to the t test.