Table I.

The nodulation process in lot1 mutant and the wild type

Infection threads were observed for the first time and counted microscopically at 3 dpi for M. loti ML001, which expresses GFP constitutively. At 7 dpi, nodules and primordia were observed for the first time and counted again. The successful nodulation ratios were then calculated by dividing the numbers of nodules and primordia by those of infection threads at 3 dpi. The means and sds are presented (n = 8). Each value for lot1 was significantly lower than that for the wild type at P < 0.01 according to the t test.

Parameter	dpi	Wild Type	lot1
Number of infection threads	3	17.7 ± 6.1	2.8 ± 2.3
	5	68.4 ± 20.0	29.2 ± 16.1
	7	153 ± 39.2	51.9 ± 21.5
Number of nodules and primordia	7	3.8 ± 1.4	0.1 ± 0.3
Successful nodulation (%)		21	4