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. Author manuscript; available in PMC: 2024 Feb 22.
Published in final edited form as: Nature. 2023 Nov 8;623(7989):1079–1085. doi: 10.1038/s41586-023-06710-4

Fig. 4 ∣. In vivo tolerability and efficacy of AM-2-19_DP2K.

Fig. 4 ∣

a, Histopathological analysis of kidneys collected 24 h post single IV dosing from mice treated with AmBisome (n = 3 per group) or AM-2-19_DP2K (n = 4 per group) (A, mixed cell infiltrate, interstitial; B, tubular basophilia, cortex; C, tubular cellular casts, cortex; D, tubular cellular casts, medulla; E, tubular degeneration and necrosis, cortex; F, tubular degeneration and necrosis, medulla; G, tubular dilatation, cortex; H, tubular protein casts, cortex; I, tubular protein casts, medulla; J, vascular congestion, medulla). b, Representative examples of in vitro efficacy of AM-2-19_DP2K and the comparator AmBisome tested at UT Health San Antonio (number of isolates in parenthesis; 100% inhibition reported). c, In vivo efficacy of AM-2-19_DP2K and the comparator AmBisome in a neutropenic disseminated candidiasis mouse model infected with C. glabrata ATCC2001 after 5 days of daily IV dosing (n = 6 per group). LOD, limit of detection. One-way ANOVA with Tukey’s multiple comparison test; ***P = 0.0008, ****P < 0.0001, **P = 0.0036. d, Efficacy of AM-2-19_DP2K and the comparator AmBisome in a neutropenic disseminated aspergillosis mouse model infected with A. fumigatus 1163 after 4 days of daily IV dosing (n = 6 per group). One-way ANOVA with Tukey’s multiple comparison test; ****P < 0.0001. e, Neutropenic invasive pulmonary aspergillosis mouse model infected with A. fumigatus ATCC 204305 after 4 days of daily IV dosing (n = 8 per group). One-way ANOVA with Tukey’s multiple comparison test; *P = 0.0117, **P = 0.0032, ****P < 0.0001. f, Neutropenic disseminated aspergillosis mouse model infected with A. terreus 49 after 10 days of daily IV dosing (n = 10 per group). One-way ANOVA with Tukey’s multiple comparison test; ***P = 0.0008, ****P < 0.0001. g,h, Survival curves of a neutropenic invasive pulmonary mucormycosis mouse model infected with R. delemar (n = 10 per group; g) and Mucor circinelloides (n = 10 per group; h) after 4 days of daily IV dose. Pairwise comparisons with the placebo using a log-rank (Mantel–Cox) test. Dosing units are milligrams per kilogram. Results are means ± s.d. Fungal burdens below the limit of detection were given a nominal value of 1 CFU g−1. Concentrations (a–h) listed reflect the mass of active ingredients.