Extended Data Fig. 3 ∣. Erg interacts with AmB through contacts between the sterol rings and conserved GPM motif.

a, A diagram summarizing AmB-Erg interactions shown in c, d, and e, highlighting ¹³C-¹³C and ¹⁹F-¹³C interactions between the Erg A and B rings and the conserved C11-C20 motif and mycosamine. b,¹³C detected ¹H-¹H polarization transfers from water to the ¹³C-AmB C13/C1’ signal for homogenized ¹³C-AmB (black line) and ¹³C-AmB:Erg complexes (blue lines). Each data point comes from one peak (n = 1). Error bars represent uncertainties from the signal-to-noise ratio of each spectrum. c, Dephasing curves (red stars for experimental data and black lines for simulated curves) from ¹³C{¹⁹F} frequency selective REDOR experiments performed on a ¹³C-AmB:C6F-Erg sample and corresponding distances calculated from the dipolar couplings. Data presented as mean +/− SD of n = 11, 5, and 10 technical replicates, respectively. Error bars indicate uncertainty from the spectrum signal-to-noise ratio. d, AmB chemical shift perturbations relative to Erg bound ¹³C-AmB for the apo AmB states, previously reported in Nat. Chem. Bio. 28, 972 (2021), and ¹³C-AmB bound to ¹³C-Erg, C6F-Erg, and Chol (SI Table 6). e, Overlay of ¹³C-¹³C 2D PAR spectra collected at 8.4 ms (blue), 12.6 ms (magenta), and 16.8 ms (cyan) mixing times obtained from a ¹³C-AmB:¹³C-Erg sample highlighting interactions between the two primary Erg ring states and AmB.