Figure 2.
Southern-blot analysis of peanut genomic DNA with the oxalate oxidase probe. BstXI-digested DNA was probed with a 32P-labeled fragment amplified by PCR from the barley oxalate oxidase cDNA. Size markers were HindIII-digested lambda DNA (sizes to the left of the blot are in base pairs). The first three lanes show hybridization of the probe to the PCR product used as the probe (470 bp), to undigested pOxOx plasmid, and to BstXI-digested pOxOx plasmid. Untransformed controls are cultivars Perry, Wilson, and NC-7. P9, P30, and P39 were Perry transformants. W3, W73, and W100 were Wilson transformants, and N6 is believed to be a NC-7 transformant.