Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 14;25(1):404–427. doi: 10.1038/s44319-023-00006-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

Figure EV5 — (A, B) Examples of mapped RNA-seq reads of differentially expressed genes (DEGs) in eIF4E1b (A) and eIF4E1c (B) RIPs performed at 1.25 hours post fertilization (hpf). (C) DEG analyses of mRNAs isolated from total lysates (inputs from RIP experiments) of 8-cell stage embryos expressing 3xflag-sfGFP tagged eIF4E1b or eIF4E1c under the control of the actb2 promoter (eIF4E1b or eIF4E1c OE, respectively). mRNAs enriched in eIF4E1b OE and eIF4E1c OE are shown in red and blue, respectively. Statistical significance was determined using Benjamini-Hochberg-corrected Wald test (DeSeq2; P value < 0.005; n = 3 biological replicates). (D) Mapped RNA-seq reads of example genes specifically upregulated in eIF4E1b (top) or eIF4E1c (bottom) OE 8-cell embryos. (E) Fraction of transcripts significantly upregulated in eIF4E1b OE (left) or eIF4E1c OE (right) embryos that were also enriched in eIF4E1b and eIF4E1c RIPs at 1.25 hpf (P value < 0.05). (F) Changes in mRNA levels (in transcripts per million, TPM) of transcripts with polyA tails containing less than 12 nucleotides during zebrafish embryogenesis according to published RNA-seq data (Bhat et al, 2023). Medians (dots) with interquartile ranges (bars) are shown.