Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 14;25(1):404–427. doi: 10.1038/s44319-023-00006-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

Figure EV3 — (A) eIF4Ea localizes to the cytosol of fixed early zebrafish oocytes. Mitochondria present in the Balbiani body (Bb) are stained with Mitotracker. Scale bars = 10 μm. (B) Confocal microscopy images of fixed transgenic embryos expressing 3xflag-sfGFP-tagged eIF4Ea, eIF4E1b and eIF4E1c (scale bars: top = 100 μm; bottom = 10 μm). eIF4E1b foci are indicated by white arrows. (C) Confocal microscopy images of squeezed (oocyte V) and activated eggs from transgenic lines expressing 3xflag-sfGFP-tagged eIF4E1c (top) and eIF4E1b (bottom). eIF4E1b foci are indicated by white arrows. (D) Colocalization experiments in zebrafish embryos expressing 3xflag-sfGFP-eIF4E1b transiently expressing P-body markers. mRNAs containing the coding sequences of dsRed-tagged P-body components (Dcp2, Ddx6 and Ybx1) were injected into 1-cell embryos. Images were taken at 3 h post fertilization. Regions enclosed by dashed boxes are shown at higher magnification on the top left (scale bars = 10 μm).