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. 2023 Dec 15;16(1):10–39. doi: 10.1038/s44321-023-00003-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV3 — (A, B) ChIP assays via designated antibodies demonstrating the recruitment of MYSM1, ERα, pCAF, CBP, p300 and the histone modification levels H3K4me1 and H3K27ac at the ERα binding site on c-Myc (A) or CCND1 (B) enhancer in MCF-7 cells upon MYSM1 depletion. (C) qPCR analysis demonstrating the eRNA expression of estrogen-induced genes in MCF-7 cells transfected with siCtrl or siMYSM1 in the presence of E2. (D, E) MNase experiments examining chromatin accessibility after digestion with 0, 20, 40, or 80 U MNase in MYSM1-depletion (D) or MYSM1-overexpression (E) MCF-7 cells. Data information: (A–C): n = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, N.S. means no significance (mean ± SD; Student’s t test).