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. 2005 Apr;137(4):1456–1462. doi: 10.1104/pp.104.055939

Figure 2.

Figure 2.

Northern-blot analysis of wild-type and gs52 transgenic L. japonicus. A 1.4-kb PCR product complementary to gs52 cDNA was used to detect gs52 mRNA expression levels (top). Lanes 1 to 6 represent the results of 20 μg total RNA from each sample. 1, Wild type; 2, 44HH T2 (double-copy antisense line); 3, 45D5 T3 (single-copy sense line); 4, 45W T2 (single-copy sense line); 5, 45B1K T3 (single-copy sense line); and 6, 45B77 T3 (double-copy sense line). A soybean actin probe was used as a RNA loading control (bottom).