Figure 3.

Nodulation results of gs52 transgenic L. japonicus plants. Compared to wild-type and line 44HH T₃ plants, sense gs52 plants had enhanced nodulation. Seeds from each line were sterilized, germinated, and grown as described in “Materials and Methods.” Three-week-old plants were equally inoculated with M. loti NZP2235 and then grown for an additional 4 weeks. The plants were scored for their nodulation phenotype 4 weeks postinoculation. Bars indicated ±se. Groups marked (a) and (b) are statistically different from one another (α = 0.05) by Student's t test. The data shown are from 1 experiment, but are representative of the 3 independent trials that were conducted (n ≥ 30 for each treatment in each trial). Line 45M T₂ (not listed in Table I) shown in this figure was a sense gs52 line that exhibited greater variation with regard to nodulation and, therefore, did not differ from the wild type. It is included here to indicate that not all sense lines showed greater nodulation, although most did. The differences are probably due to positional effects of the various T-DNA insertions.