Table I.
Segregation analysis of gs52 L. japonicus plants
Plants were germinated on filter paper and then transferred to selection medium (see “Materials and Methods”) and scored for resistance or sensitivity to G418. Transgene copy number was determined by Southern blotting and is listed in column 4 of the table. S, Sense copy of gs52; AS, antisense copy of gs52.
| Line | Resistant:Sensitive | Segregation | Southern Results |
|---|---|---|---|
| 45 W T2 | 34:9 | 3.8:1 | 1 Copy |
| 45D5 T3 | 68:26 | 2.8:1 | 1S |
| 45B1K T3 | 51:15 | 3.4:1 | 1S |
| 45B77 T3 | 62:0 | Homozygousa | 2S |
| 45A7 T3 | 75:0 | Homozygous | 2S |
| 45B38 T3 | 64:0 | Homozygous | 2S |
| 45B1.2 T3 | 92:0 | Homozygous | 2S |
| 45B1H T3 | 60:0 | Homozygous | 2S |
| 45B35 T3 | 22:0 | –b | 2S |
| 45B1I T3 | 100:0 | Homozygous | 2S |
| 45B16 T3 | 53:0 | Homozygous | 2S |
| 45B37 T3 | 73:0 | Homozygous | 2S |
| 44HH T2 | 99:0 | Homozygous | 2AS |
a
All plants were G418 resistant and were homozygous for at least one of the two inserts.
b
Inconclusive.