Table 1. ITC measured thermodynamic parameters.
Kd, dissociation constant; ND, not detected. The experiments were performed three times for those with measurable binding and two times for those with undetectable binding, and the repeated experiments yielded similar results; for each case, only the result of one representative experiment is presented.
| dSAMTOR | Ligand | n value | Kd (μM) | ΔH (kcal mol−1) | TΔS (kcal mol−1) |
| WT | SAM | 0.75 ± 0.08 | 10.70 ± 0.88 | −2.12 ± 0.05 | 4.66 |
| WT | SAH | 0.81 ± 0.09 | 8.70 ± 0.92 | −9.94 ± 1.48 | −2.99 |
| Δ1-64 | SAM | 0.91 ± 0.01 | 6.78 ± 0.75 | −1.16 ± 0.01 | 5.89 |
| Δ1-64 | SAH | 0.67 ± 0.04 | 13.10 ± 0.80 | −14.70 ± 1.05 | −8.08 |
| V66W/E67P | SAM | 0.88 ± 0.02 | 9.47 ± 0.81 | −3.01 ± 0.08 | −3.84 |
| V66W/E67P | SAH | 1.02 ± 0.02 | 11.30 ± 0.37 | −5.09 ± 0.12 | −1.66 |
| R73A | SAH | ND | ND | ND | ND |
| D150A | SAH | ND | ND | ND | ND |
| D162A | SAH | ND | ND | ND | ND |
| L151A | SAH | ND | ND | ND | ND |
| L163A | SAH | ND | ND | ND | ND |
| L197A | SAH | ND | ND | ND | ND |
| Y201A | SAH | 0.74 ± 0.02 | 67.30 ± 6.03 | −2.89 ± 0.17 | 2.80 |
| M202A | SAH | ND | ND | ND | ND |
| F135A | SAM | 0.79 ± 0.01 | 6.46 ± 0.40 | −5.24 ± 0.11 | −1.84 |
| F135A | SAH | 0.80 ± 0.02 | 4.76 ± 0.71 | −8.52 ± 0.51 | −1.26 |