FIG. 3.

Interaction of c-Rel and CCR.SVNLS with wild-type or mutant IκBα. Extracts from Cos-7 cells transfected with CMV vectors expressing c-Rel (lanes 2 to 10) or CCR.SVNLS (lanes 11 to 19), alone (lanes 2 and 11) or together with expression plasmids for IκBα (lanes 3 and 12) or IκBα mutants (lanes 4 to 10 and lanes 13 to 19), were immunoprecipitated with an anti–c-Rel antibody. Complexes were resolved on 12% polyacrylamide–SDS gels and analyzed by immunoblotting with antibodies specific for c-Rel (top panels) or IκBα (bottom panels). The positions of c-Rel, CCR.SVNLS, and wild-type or mutant IκBα proteins are indicated. Control immunoblots verified that equivalent amounts of wild-type and mutant IκBα proteins were expressed in the cells (data not shown).