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. 2005 Apr 21;102(18):6273–6278. doi: 10.1073/pnas.0409892102

Fig. 3.

Fig. 3.

Transfection analysis and initial characterization of positive feedback vectors. (A) Reporter assays with different configurations of promoter elements controlling the expression of the dicistronic mRNA. Four promoter elements were tested in the promoter/R/Gal4 vector: the SV40 promoter, a minimal TATA box, a minimal TATA box with one UAS, and a minimal TATA box with four UASes. The 4XUAS/R/Gal4 vector was also tested in a cotransfection experiment along with the SV40/Gal4 expression vector. (B) Reporter assays with different control ICSes. Sequences tested in this study were a no-insert control (-), a transcriptional enhancer (p22), and a minimal TATA box. CHO cells were transfected with the indicated constructs, and the cells were harvested 2 days later. Reporter activities were determined. For each case, the results of two independent experiments, each presented as a shaded bar, are shown in the histogram.