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. 2024 Jan 29;236(2):73–79. doi: 10.1055/a-2233-1243

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The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 1 — Whole lung lavage of hereditary pulmonary alveolar proteinosis (A) Tissue section of the distal lung compartment to assess the proteinosis phenotype within the alveoli. Tissue sections are typically stained by the Periodic acid-Shiff (PAS) method to stain for muco-like substances in the lung such as glycoproteins, glycolipids and others. Picture shows an extensive proteinosis by alveoli filed with surfactant material. (B) Photo showing the recovery of sequential lung lavage (WLL) batches in a child with herPAP. Each lavage recovery is numbered from the left to the right (I to VII), showing the overall improvement demonstrated by a decrease turbidity. (C) Highlighting examples from WLL recoveries demonstrating the decrease in muco-like substances which is highlighted by the white brackets. Three pictures are shown from first (I), third (III) and 6th (VI) lavage.