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. 2005 Apr 19;102(18):6467–6472. doi: 10.1073/pnas.0501912102

Fig. 2.

Fig. 2.

Mast cell-derived TNF is required for optimal T cell activation mediated by IgE/Ag-stimulated mast cells. (A) Proliferation of CD3+ T cells cocultured with WT or TNF-/- BMCMCs. *, P < 0.05 vs. corresponding values for any other group in that box; †, P < 0.05 vs. corresponding values for T cells plus TNF-/- BMCMCs. (B) Proliferation of CD3+ T cells cocultured with IgE-sensitized WT or TNF-/- BMCMCs in the presence of DNP-HSA and various concentrations of rmTNF. rmTNF restored the reduced T cell proliferation observed in experiments by using TNF-/- instead of WT mast cells. *, P < 0.05 vs. corresponding values for any other group in that box; †, P < 0.05 vs. corresponding values for T cells plus TNF-/- BMCMCs. (C) Proliferation of CD3+ T cells cocultured with WT or TNF-/- BMCMCs at low (Upper) or high (Lower) cell concentrations. [3H]thymidine incorporation was measured at 72 h at low, and at 48 h at high, cell concentrations. †, P < 0.05 vs. corresponding values for T cells plus TNF-/- BMCMCs; *, P < 0.05 vs. corresponding values for BMCMCs alone. Data in A-C are mean ± SD (A and B) or + SD (C) (triplicate wells) and show representative results from at least three experiments using at least two different batches of BMCMCs.