Fig. 4.

Lineage tracing confirms the overactivation and subsequent exhaustion of NSC pool with impaired neurogenesis after ICH or iron injection (A) Schematic diagram and experimental timeline for the tamoxifen-induced Nestin:CreER^T2-mediated lineage tracing in mice. (B) Representative images of brain section stained with GFAP, MCM2, DCX and genetically labeled tdTomato in the DG of ICH group and FeCl₂ injected group at 7 days after ICH or FeCl₂ injection. Scale bar = 20 μm. (C) Quantification of numbers of rNSCs (GFAP⁺Td⁺), proliferating rNSCs (MCM2⁺Td⁺) and newborn neurons (DCX⁺Td⁺) from B. (n = 6 for each group). (D) Representative images of brain section stained with GFAP, MCM2, DCX, NeuN and genetically labeled tdTomato in the DG of ICH group and FeCl₂ injected group at 90 days after ICH or FeCl₂ injection. Scale bar = 20 μm. (E) Quantification of numbers of rNSCs (GFAP⁺Td⁺), proliferating rNSCs (MCM2⁺Td⁺), newborn neurons (DCX⁺Td⁺) and adult-born neurons (NeuN⁺Td⁺) from D. (n = 6 for each group). (C, E) **P < 0.01; ***P < 0.001 vs corresponding Sham. NS = no significance. Each experiment was repeated at least 3 times independently.