Skip to main content
. 2024 Feb 13;71:103086. doi: 10.1016/j.redox.2024.103086

Fig. 5.

Fig. 5

Elevated ROS level mediated the effect of iron accumulation on NSC pool and neurogenesis after ICH (A) Schematic diagram of the ICH model and experimental timeline for ROS analysis of ICH mice. (B) Representative images of brain section stained with ROS and genetically labeled Nestin-GFP in DG of Sham, ICH, ICH + DFO and ICH + NAC group at 7 days after ICH. Scale bar: 5 μm. (C) Quantification of ROS fluorescence intensity from B. (n = 6 for each group). (D) Experiments timeline for cell proliferation analysis and neurogenesis trace by an EdU pulse chase in ICH mice with or without NAC treatment at 7 days after ICH. Scale bar = 20 μm. (E) Representative images of brain section stained with rNSCs (Sox2, EdU and GFAP) in the DG of ICH mice with or without NAC treatment at 7 days after ICH with or without NAC treatment. Scale bar = 20 μm. (F) Quantification of numbers of Sox2+GFAP+EdU+ cells from E. (n = 12 for each group). (G) Representative images of brain section stained with NeuN, EdU and DAPI in the DG of ICH mice with or without NAC treatment at 90 days after ICH. Scale bar = 20 μm. (H) Quantification of numbers of NeuN+EdU+ (adult-born neurons) cells from G. (n = 12 for each group). (I) Experiments timeline for behavioral tests of ICH mice with or without NAC treatment. (J) Schematic diagram of novel object location and novel object recognition test. (K) Quantification of the ratio of exploration time on novel objective location and novel object recognition in ICH + Vehicle and ICH + NAC mice at 90 days after ICH. (n = 6 for each group). (L) Representative images of the swimming path of ICH + Vehicle and ICH + NAC mice during the probe trial test in Morris water maze test at 90 days after ICH. (M) Platform-crossing times of ICH + Vehicle and ICH + NAC during probe trial test. Escape latency of probe trial test of ICH + Vehicle and ICH + NAC mice. Duration of stay in the platform quadrant. N = 6 for each time point in each group. (C) **P < 0.01; ***P < 0.001 vs corresponding ICH. (F, H, K, M) *P < 0.05; **P < 0.01; ***P < 0.001 vs corresponding ICH + Vehicle. Each experiment was repeated at least 3 times independently.