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. 2024 Feb 13;71:103086. doi: 10.1016/j.redox.2024.103086

Fig. 7.

Fig. 7

Conditional overexpression of Itga3 attenuates NSC pool exhaustion and cognitive impairment following ICH (A) Mouse strain and scheme of experimental design. (B) Representative images of brain section stained with GFAP, MCM2, DCX, NeuN and genetically labeled GFP in the DG of Sham, ICH + Scramble and ICH + Itga3CDS group at 90 days after surgical manipulations. Scale bar = 20 μm. (C) The number of rNSCs (GFP+GFAP+), proliferating rNSCs (GFP+MCM2+), newborn neurons (GFP+DCX+) and adult-born neurons (GFP+NeuN+) cells at 90 days after ICH, respectively. Conditional overexpression of Itga3 in ICH mice alleviates the decreased neurogenesis induced by ICH. (n = 4 for each group). (D) Representative images of the swimming path of Sham, ICH + Scramble and ICH + Itga3CDS mice during the probe trial test in Morris water maze test at 90 days. Scale bar: 100 μm. (E) Platform-crossing times, escape latency, duration of stay in the platform quadrant of probe trial test from Sham, ICH + Scramble and ICH + Itga3CDS group. (n = 6 for each group). (F) Schematic drawing of novel object location and novel object recognition test. (G) Quantification of the ratio of exploration time on novel objective location and novel objective object in Sham, ICH + Scramble and ICH + Itga3CDS mice. (n = 6 for each group). (C, E, G) *P < 0.05; **P < 0.01; ***P < 0.001 vs corresponding ICH + Scramble. Each experiment was repeated at least 3 times independently.