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. 2024 Feb 22;15:1637. doi: 10.1038/s41467-024-45525-3

Fig. 1. ZNF598 is upregulated under mitochondrial stress conditions in vitro and in vivo.

Fig. 1

a Western blot analysis and quantification of ZNF598 protein level in HeLa cells treated with EBSS (16 h), Torin1 (0.5 μM, 24 h), and CCCP (10 μM, 24 h). b Western blot analysis and quantification of ZNF598 protein level in HeLa cells treated with rotenone (5 μM, 24 h) or CCCP (10 μM, 24 h). Western blot analysis of HA-tagged ZNF598 (c), Pelo (d), Rack1 (e), and ABCE1 (f) proteins in the muscle tissue of flies treated with rotenone (250 μM) or CCCP (100 μM) for 7 days. Values under the blot show normalized protein levels relative to untreated sample in this figure and all subsequent ones. g Western blot analysis of the indicated proteins in total and mitochondrial fractions isolated from striatum tissues of normal and MPTP-indued mice. Data are representative of at least three biologically independent experiments (mean ± SD), n = 3 in (a–c), and (f), n = 4 in (g). Representative blot is from three independent experiments with similar results in (a–f). p values in (a–c), and (f) were calculated by one-way ANOVA (Tukey’s test). p values in (g) were calculated by unpaired two-tailed t test. Source data are provided as a Source Data file.