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. 2023 Dec 27;17(4):567–587. doi: 10.1016/j.jcmgh.2023.12.012

Figure 7.

Figure 7

CD promotes cell apoptosis through TGF-β1 signaling. (A) Brightfield images of AML12 cells treated (72 h) with CD alone, LY2157299 (10 μmol/L), or their combination. Untreated cells served as control. Scale bars: 50 μm. (B) The viability of AML12 cells treated for 72 hours with/without CD ± 10 μmol/L LY2157299 was examined by MTT assay. (C) Cleaved caspase signals were tested by the caspase–Glo 3/7 assay kit on AML12 cells subjected (or not; control) to CD treatment ± 10 μmol/L LY2157299 for 72 hours. (D) Western blot of cleaved and total caspase 3/7 in AML12 cells subjected to CD treatment with/without 10 μmol/L LY2157299 for 72 hours. For RT-qPCR, mouse Ppia was used as endogenous control. Bars represent means ± SD (n = 3). ∗P < .05, ∗∗P < .01, NS = P > .05. For Western blot, glyceraldehyde-3-phosphate dehydrogenase was used as a loading control. Quantification of protein expression was performed using ImageJ (National Institutes of Health). Images were chosen representatively from 3 independent experiments. Con, control; RLU, relative light unit.