FIG. 2.

Structure and expression of the MT-NK1 transgene. (A) Schematic representation of the 19-kb ClaI/SstII DNA fragment used to generate NK1-transgenic mice. The expression construct included the mouse NK1 cDNA (black box), the MT-1 promoter and human growth hormone gene polyadenylation site (hGH PA), and the 5′ and 3′ flanking sequences containing the locus control regions (LCR) of the mouse MT gene (white boxes). The region used as a probe for the Northern analysis whose results are shown in panel B is underlined. (B) Comparison of the expression levels of the MT-NK1 and MT-HGF/SF transgenes. The arrow indicates the position of the 0.8-kb MT-NK1 transgene transcript in NK1 line MN21 mice; the arrowhead shows the position of the 2.4-kb HGF/SF transgene transcript in HGF/SF-transgenic line MH19 mice (35). Filters were stripped and rehybridized to a probe for GAPDH to control for differences in loading and transfer of RNAs. s.i., small intestine; sk. muscle, skeletal muscle; mam. gl., mammary gland.