FIG. 3.

α-NAC/1.9.2 interacts with GAL4/VP-16. Results from gel mobility shift assays with purified recombinant proteins with a labeled 17-mer GAL4 probe are shown. GAL4(DBD-1–147) is a fusion protein between GST and the DBD (residues 1 to 147) of GAL4. GAL4(1–147)-VP-16 is the same as GAL4/VP-16. α-NAC denotes the purified full-length α-NAC/1.9.2 protein. Preimmune sera (lanes 7 and 8) and antibodies raised against α-NAC/1.9.2 (anti-α-NAC [lanes 8 and 10]) were added to some binding reaction mixtures.