FIG. 5.

Interactions between α-NAC/1.9.2 and TBP. (A) Affinity chromatography of crude nuclear extracts eluted with a salt step gradient. The immunoblot (lanes 3 to 15) was probed with the anti-TBP antibody. Recombinant human TBP (rTBP) was migrated in lane 15. (B) Control affinity chromatography column loaded with the GST moiety alone. The same crude nuclear extract used in panel A was passed through the column. Immunoblotting was with the anti-TBP antibody. In, input (1/100 of total material loaded on column); F.T., flowthrough. Lanes 1 and 2 contained Coomassie blue-stained molecular mass (kilodaltons) markers (M) and input material, respectively.