FIG. 6.
Protein-protein interactions between α-NAC/1.9.2 and GAL4/VP-16 and between α-NAC/1.9.2 and TBP. Results of a far-Western protein blot assay show the interaction of biotinylated GST-1.9.2 with TBP (lanes 2 and 3) and GAL4/VP-16 (lane 4). The fusion protein GST-1.9.2(111–215) (Mr, 39,400) served as a negative control in this assay (lane 1). Asterisks point to the proper molecular size of GST-TBP (lane 2), cleaved TBP (lane 3), and GAL4/VP-16 (lane 4). Molecular mass (kilodaltons) markers are indicated in the left-hand lane.