FIG. 7.

α-NAC/1.9.2 is associated with TBP in vivo. Immunoprecipitation and immunoblotting assays demonstrating the specific interaction of α-NAC/1.9.2 with TBP in crude nuclear extracts. Immunoprecipitation reactions were performed with preimmune sera [lanes 3, 6, and 9]), anti-TBP antibodies (A [lanes 2, 5, and 8]), anti-αNAC antibodies (A [lanes 1, 4, and 7]), or anti-RNA polymerase II (pol. II) antibodies (B [lanes 1 to 4]). The immunocomplexes were then analyzed by immunoblotting with the antibodies indicated in the upper portion of each panel. TBP was specifically associated with the anti-αNAC immunocomplexes (A, lane 7). Similarly, α-NAC/1.9.2 was specifically associated with the anti-TBP immunocomplexes (A [lane 5]). M, molecular mass (kilodaltons) markers (M). IgG, immunoglobulin G.