FIG. 10.
CBFα2 activates the IgH μ enhancer in nonlymphoid cells in cooperation with Ets-1. HeLa cells were transfected with reporter plasmids containing the (μ70)2 enhancer along with expression plasmids for PU.1 (2 μg) and Ets-1 (2 μg) (bar 1) and for PU.1 (1 μg), Ets-1 (1 μg), and CBFα2 (2 μg) (bar 2) or with an empty pEVRF2 expression plasmid as carrier DNA (bar 6). The transcription activation abilities of PU.1 (1 μg), Ets-1 (1 μg), and CBFα2 (2 μg) were also tested by cotransfecting these reporter plasmids with binding site mutant versions of the (μ70)2 enhancer, μA− (bar 3), μE3− (bar 4), and μB− (bar 5). Cells were harvested 2 days after being transfected, and CAT analysis was performed by ELISA. Results shown are the averages of at least two transfections carried out in duplicate. Error bars indicate the average deviations of the data.