FIG. 3.
The minimal murine and human IgH μ enhancers activate transcription comparably. Reporter plasmids (5 μg) containing dimeric murine μ70 [(μ70)2], murine μE3 mutant (μE3−), and human [h(μ51)2] enhancers were transfected into S194 plasma cell lines, and CAT assays were performed by ELISA as described in Materials and Methods. CAT enzyme activity is shown on the y axis as the percentage of the amount of CAT enzyme obtained with the dimeric murine μ70 reporter. Results show the averages of at least two transfections carried out in duplicate. Δ56 refers to an enhancerless reporter. Error bars indicate the average deviations of the data.