FIG. 3.

T antigen activates transcription of the U6 RNA gene. CV-1 cells were transfected with the U6 RNA producing plasmid pSP72-U6 sense, along with 0, 1, 3, or 5 μg of plasmids expressing full-length large T antigen and small t antigen (WT T+t), full-length T antigen alone (WT T only), or various mutants of T antigen (Table 1). The α-globin RNA producing plasmid pα4X(A+C) was used as the internal control. At 42 h posttransfection, the total RNA was harvested and U6 RNA and α-globin RNA production was analyzed by RNase protection as described in Materials and Methods. The protected U6 (184 nucleotides) and α-globin (132 nucleotides) bands are indicated.