Figure 1.

PDH activity is rescued by supplementation of C(18:2)₄CL.A, phosphorylated PDH (p-PDH) levels were assayed in mitochondria obtained from WT and TAZ-KO myoblasts. Mitochondria were incubated on ice for 2.5 h with the indicated CL species. The signal intensities of protein bands were quantified using ImageJ software (bottom panel). The protein band was normalized to NDUFB6 and quantified relative to the WT control-treated sample. B and C, mitochondria from WT and TAZ-KO myoblasts were treated with or without C(18:2)₄ CL, and PDH activity was assayed as described in “Experimental procedures”. Data points represent mean ±S.D. (error bars) for each individual biological replicate of each group. ∗ 0.01<p < 0.05, ∗∗ 0.001<p < 0.01, ∗∗∗ <p < 0.001.