Figure 4.

The binding of PDP1 to PDH is reduced in TAZ-KO cells.A, WB analysis of the bait-prey pull-down assay. B, cell lysates from WT and TAZ-KO cells were subjected to WB analysis using the indicated antibodies. C, co-immunoprecipitation assay for PDP1, PDH-E1, and PDH-E2 protein interaction. The indicated myoblast lysates were immunoprecipitated using the antibodies specified on the left side of the figure, and eluted protein was subjected to WB analysis by probing for the specific proteins listed on the right side. The negative control lane represents WT lysate passed through empty beads lacking primary antibodies.