Figure 5.

PDP1 activity is decreased in TAZ-KO cells. Increasing amounts of recombinant PDP1 protein were incubated with WT or TAZ-KO lysates. PDP1 eluted from this mixture was subsequently incubated with PDH complex for 1 h. The dephosphorylation activity of PDP1 was evaluated by assaying phosphorylated PDH (p-PDH) using WB analysis. p-PDH levels were quantified by dividing the amount of p-PDH signal by total PDH-E1 in each sample, and values were normalized relative to the first lane in each group (containing the lowest concentration of eluted PDP1).