Figure 6.

TAZ-KO mitochondria exhibit decreased calcium levels. WT and TAZ-KO cells were preloaded with Fluo-4 AM and TMRM before permeabilization with 50 μg/ml (w/v) digitonin and 1 μM thapsigargin. Steady-state quantification of Fluo-4 AM (A) and TMRM (B) signals in WT and TAZ-KO mitochondria was performed using Image J software. n = 31. Subsequently, WT and TAZ-KO mitochondria were injected with 5 mM CaCl₂ (100 μl) into the 250 μl imaging solution at the indicated time points (indicated as each ∗, with the resulting effective [Ca²⁺] listed below). Fluo-4 AM (C) and TMRM (D) density were quantified over time. Data points represent mean ±S.D. (error bars) for each individual biological replicate of each group. ∗∗ 0.001<p < 0.01, ∗∗∗ <p < 0.001.