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Activation of DRAF1 in Pkr0/0 MEFs. Wild-type (Pkr+/+) MEFs or PKR-deficient (Pkr0/0) MEFs were either untreated (lanes 1 and 4) or infected with NDV for 3 h (lanes 2, 3, 5, and 6). Nuclear extracts were prepared and analyzed by an EMSA with a radiolabeled ISRE probe. The specificity of the protein-DNA complexes was shown by competition with a 100-fold excess of unlabeled ISRE oligonucleotide (lanes 3 and 6).
