FIG. 5.

Corepressor overexpression prevents 8-bromo-cAMP-mediated potentiation of PR transcriptional activity. HeLa cells were transiently transfected as indicated previously with expression vectors for PR alone or for PR along with NCoR (A) or SMRT (B). After transfection, the cells were incubated in the absence or presence of 8-bromo-cAMP (8Br) (1 mM) with either no hormone (NH) or designated ligand R5020 (10 pM to 1 nM), RTI-020 (10 pM to 1 nM), or RU486 (1 to 100 nM) for 48 h and subsequently harvested for luciferase and β-galactosidase assays. Luciferase data were normalized to the β-galactosidase activity, and the data are represented as the fold induction over the response in the absence of hormone. The data presented are representative of multiple independent experiments.