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. Author manuscript; available in PMC: 2024 Feb 23.
Published in final edited form as: Exp Neurol. 2019 Nov 18;325:113119. doi: 10.1016/j.expneurol.2019.113119

Fig. 6. CHI induces Nestin+ cells in the hippocampus.

Fig. 6.

(A) Injury-induced GFP+ cells in the hippocampus were determined by immunostaining in a mouse model of closed head injury (CHI). Confocal images show GFP+ cells co-labeled with NSPC marker Nestin (purple) in the hippocampus in coronal sections at 2 DPI. Arrowheads indicate co-labeled cells. Dash line boxed region shows an example of Nestin+/GFP+ co-labeled cells in separate image channels and in a higher magnification with orthogonal views of Z-stack. Quantification shows an increase in the number of Nestin+/GFP+ cells (2.58 ± 0.53% in CHI vs. 0.36 ± 0.12% in sham; p < .01) out of all the cells in the ipsilateral cerebral cortex of injured mice compared to sham mice. Mean ± SEM, n = 6. Examples of photomicrographs of anti-Nestin antibody staining in the SVZ of the contralateral (B) and ipsilateral (C) brain regions of the CHI animal. Scale bars = 20 μm.