FIG. 9.

Inhibition of p110 by p85 in mammalian cells. HEK 293T cells were transfected with GST-p110α in the presence of control vector or p85 expression vector as indicated. After 48 h at 30°C, the cells were lysed and protein and lipid kinase activities were determined. The activities were normalized to the activity of GST-p110α. All determinations were performed in triplicate, and the data are representative of four separate experiments.