Individual target points are shown for CASP14. The two targets with the lowest agreement with experiment are colored blue are NMR structures and red point represent the model of a a subunit of a cryo-EM-derived large heteromeric structure. The agreement metric, GDT_TS, is a multi-scale indicator of the closeness of the Cα atoms in a model to those in the corresponding experimental structure, and is reported as a percentage, ranging from 0 to 100. Because of experimental errors and artifacts, models with GDT-TS >90 are considered compatible with experiment in backbone accuracy (65). Target difficulty is based on sequence and structure similarity to other proteins with known experimental structures. (Reproduced from reference (66) with permission).