FIG. 6.

Whole-cell protein tyrosine phosphorylation in Jurkat subclones infected with Syk- or ZAP-encoding vaccinia virus. Wild-type (WT) Jurkat, J.CaM1, or P116 cells were infected with control vaccinia virus strain WR or with recombinant vaccinia virus encoding ZAP-70 or Syk. Three hours after infection, cells were stimulated for 1 min with medium only or with anti-CD3 antibody cross-linked with goat anti-mouse IgG. Detergent-soluble proteins were resolved by SDS-PAGE and immunoblotted with antiphosphotyrosine MAb.