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. 2024 Feb 10;40(2):btae078. doi: 10.1093/bioinformatics/btae078

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© The Author(s) 2024. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — mBARq recapitulates the evolutionary trajectories of barcoded isogenic E. coli strains over 420 generations. (A) Each panel shows the frequency trajectories for barcoded E. coli populations cultured without an antibiotic over 420 generations. Each colored band corresponds to a unique lineage, with its vertical width indicating its frequency at a particular time point. Only the 20 most abundant lineages are shown. (B) Correlation between average frequencies for the 20 most abundant lineages from each of the replicates as calculated from mBARq generated counts and the original analysis. (C) Final frequencies (i.e. at generation 420) of the 20 most abundant barcodes as calculated from mBARq counts and in the original analysis.