Fig. 4.

repNP + repGc-FL confers equivalent protection to repNP + repGPC vaccination. Mice vaccinated with sham RNA or repNP RNA plus repGc-sol, repGn-sol, repGc-FL, or repGPC RNA were treated with MAR1-5A3 antibody on D0 and infected with a lethal dose of 100 TCID₅₀ CCHFV strain UG3010. Mice (N = 8) were monitored daily for (a) weight loss and for (b) survival until D14 p.i. On D5 p.i., groups of mice (N = 6) were evaluated for control of (c) viral genome copies via qRT-PCR and (d) infectious virus via TCID₅₀. Dashed lines indicate limit of detection. Sham and repGPC + repNP group data is duplicated from Figs. 1 and 2 for comparison. Significance was calculated using one-way ANOVA; ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. Data shown as mean plus standard deviation.