FIG. 2.

Repair of G · G, T · G, A · G, and G · A mispairs by Drosophila wild-type and mei-9 mutant extracts. Extracts were prepared from Oregon-R strain (wild-type) young adults and from flies heterozygous (het) and homozygous (hom) for mei-9 alleles mei-9^AT2 and mei-9^a, as indicated. Results are expressed as specific activity (percent repair efficiency/minute/microgram of protein) and are based on counts of several hundred to several thousand plaques per assay. Reaction mixtures contained 0.5 μg of protein and were incubated for 2 min at 37°C, which is within the approximately linear range of the assay (Fig. 1C). The data are plotted as means ± standard errors of two to four independent measurements. All mispairs were efficiently repaired by a mixture of wild-type and mei-9 mutant extracts (data not shown).