TABLE 3.
Measurement of specific activities of extracts for the repair of G · G heteroduplex
| Extract | Sp act (% repair/min/μg of protein)
|
|
|---|---|---|
| Extract 1 | Extract 2 | |
| Drosophila embryos | 24.2 | 16.3 |
| Drosophila young adult | 19.2 | 20.9 |
| Drosophila old adult | 22.5 | 21.7 |
| Drosophila young cephalothorax | 47.1 | NDa |
| Drosophila old cephalothorax | 44.7 | ND |
| HeLa | 0.14 | 0.12 |
| HeLa (using Drosophila extract protocol) | 0.08 | ND |
| X-ray treatment | ||
| Controlb | 7.5 | 12.4 |
| Treated | 44.0 | 69.2 |
a
ND, not determined.
b
Small-scale preparation of whole-fly extracts generally yields about half of the specific activity of large-scale preparations (see Materials and Methods).